
Cell disruption is an important step in recombinant protein production procedures. We offer laboratory, bench and industrial-scale high-amplitude ultrasonic processors, which can be used for efficient cell disruption. The processors are based on our patented Barbell Horn® Ultrasonic Technology (BHUT).
Overview
Cell disruption (lysis) is an important step in recombinant protein production procedures. Ultrasonic cell disruption (lysing with ultrasound) has been the laboratory-scale method of choice for this application for several decades. The process requires high ultrasonic amplitudes to be applied to cell suspensions, producing extreme shear forces. The shear forces are the result of intense ultrasonic cavitation, which creates violently and asymmetrically imploding vacuum bubbles and causes micro-jets that rupture cell walls. However, due to limitations of conventional ultrasonic technology, this method’s industrial implementation has not been possible without reducing ultrasonic amplitudes, diminishing the intensity of cavitation-generated shear forces and, therefore, compromising the efficiency of the lysis process. These limitations have been overcome with BHUT, which permits constructing industrial-scale ultrasonic cell disruptors with the same lysis efficiency as the laboratory devices, while offering much higher productivities.
Case Studies & Application Reports
Ultrasonic Disruption of Yeast Cells
Microalgal Oil Extraction and Transesterification
Cell Disruption for Recombinant Protein Production
Blog Posts
04/28/20
Vaccine and Antiviral Drug Production with ISM Ultrasonic Processors
Recent years have seen major breakthroughs in how vaccines and antiv... Read more...
10/22/16
Cannabis Concentrates: Medical Significance and Extraction Methods
The cannabis (marijuana, hemp) plant has been used for medicinal pur... Read more...
01/02/16
Acoustic Cavitation: The Driving Force Behind Ultrasonic Processing
Liquids exposed to high-intensity ultrasound can undergo acoustic ca... Read more...
Customer Testimonials
Dominic Notter, Matrix of Life
“ISM supplied us with their industrial-scale ultrasonic processor, ISP-3000, which we have been using to make nutraceutical and cosmetic products, as well as for essential oil extraction and several similar applications for over a year. We are pleased to say that with this processor, the efficiency of plant oil extraction, emulsification and solids’ dispersion is superb, enabling us to make products comprising dozens of different oils, solid powders and water. The products seem to remain permanently stable and have many other enhanced properties: our customers consistently report superior taste, texture and overall performance. We look forward to using this technology for developing many new processes in the near future.”